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. Author manuscript; available in PMC: 2022 Apr 5.
Published in final edited form as: J Neurochem. 2008 Feb 1;105(5):1683–1699. doi: 10.1111/j.1471-4159.2008.05262.x

Fig. 5.

Fig. 5

Cholesterol depletion does not alter DAT membrane raft association. rDAT-LLCPK1 cells were treated with vehicle (a) or 5 mM MβC (b) for 30 min at 37°C. Cells were solubilized with 0.1% Triton X-100 followed by sucrose gradient centrifugation and fractions were immunoblotted for DAT or Caveolin. (A and B) Representative immunoblots with sucrose concentrations indicated at the top. (C and D) Quantification of DAT (c) and caveolin (d) protein distribution after vehicle (gray bars) or MβC (black bars) treatment (means ± SE of two or more independent experiments, **, p < 0.01).