Fig. 8.
Irreversible cocaine binding to DATs in raft and non-raft membranes. rDAT-LLCPK1 cells were irreversibly labeled with the cocaine photoaffinity analog [125I]RTI 82 at 4°C and cells were solubilized with 0.1% Triton X-100 followed by sucrose gradient centrifugation. (a) Autoradiograph of DATs immunoprecipitated from each fraction. (b) Quantification of [125I]RTI 82 photolabeled DAT intensities. Results are representative of two independent experiments.