Figure 2.

Fecal transplant shifts both gut and mammary microbiome. 16S sequencing of DNA isolated from fecal and breast tumor samples. A. Proportional abundance of Bacteroidetes and Firmicutes phyla in fecal samples collected from the different treatment groups. B. Volcano plots comparing microbiota population shifts in fecal samples collected from lard diet-fed and lard diet-fed mice administered weekly fecal transplants with feces from control diet-fed mice. C. Volcano plots comparing microbiota population shifts in fecal samples collected from control diet-fed and control diet-fed mice administered fecal transplants from the lard diet group. D. Total operational taxonomic units (OTU) counts in mammary tumors. Tumoral OTU counts of Massilia_unclassified (E), Enterobacteriaceae_unclassified (F), and Pseudomonas veronii (G) were identified in tumors from the different treatment groups. n=5; *p<0.05 relative to control diet.