Bacteria localization in human tissue. A. Primary breast tumors, breast cancer lymph node metastases, and tumor-adjacent normal mammary gland tissue were stained with an antibody against gram-positive bacteria to indicate the presence of bacteria in human patient samples. B. Primary breast tumors, breast cancer lymph node metastases, and tumor-adjacent normal mammary gland tissue were stained with an antibody against LPS. C. LTA and LPS stained tissue were quantified. n=10–50. D. Breast tumor tissue microarray was stained against CD45 (blue), LTA (red), and pan-cytokeratin (green). E. Bacteria laden cells correlates with tumor-infiltrating leukocyte content (n=20; r=0.8945; p<0.001).