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. 2022 Apr 4;12:42. doi: 10.1186/s13578-022-00776-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 13 — Overexpression of UBIAD1 reduced ferroptotic neuronal death by attenuating Golgi apparatus dysfunction caused by OGD/R insult in vitro. BFA and GSH were pretreated with UBIAD1-OE and UBIAD1-siRNA neurons in corresponding experimental groups before OGD/R insult. A–D and M–P The protein expression of GPX4, FTH1, and ASCL4 in various experimental groups as detected using western blotting. E, F and Q, R Content of Fe²⁺ and total iron in various experimental groups as evaluated using iron assay kit. G and S GPX4 activity in various experimental groups. H, I and T, U 12-HETE and 15-HETE levels in various experimental groups as determined using ELISA assay kits. J and V LPO level in various experimental groups. K and W The level of LDH in various experimental groups as evaluated using LDH assay kit. L and X Lipid peroxidation in neurons based on BODIPY 581/591 C11 staining (Scale bar = 50 μm). n = 3, all data are expressed as the mean ± SD, *P < 0.05, **P < 0.01; OGD/R + vector -UBIAD1-OE group vs. OGD/R + vector-UBIAD1-OE + BFA group, OGD/R + UBIAD1-OE group vs. OGD/R + UBIAD1-OE + BFA group; OGD/R + vector-UBIAD1-siRNA group vs. OGD/R + vector-UBIAD1-siRNA + GSH group, OGD/R + UBIAD1-siRNA group vs. OGD/R + UBIAD1-siRNA + GSH group