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. 2022 Apr 4;12:42. doi: 10.1186/s13578-022-00776-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Expression and location of UBIAD1 in response to cerebral I/R injury in vivo and vitro. A–C The protein and mRNA expression of UBIAD1 in neurons as detected with western blotting and PCR assay. D–F The protein and mRNA expression of UBIAD1 in brain tissues as detected with western blotting and PCR assay. G, H Protein expression of UBIAD1 in brain tissues as evaluated through immunohistochemical staining (Scale bar = 50 μm). I Confocal images showing co-localization of UBIAD1 (red) with neuron marker NeuN (green), astrocytes marker GFAP (green) microglia marker Iba-1 (green) (Scale bar = 25 μm). n = 3 for in vitro, n = 6 rats/group for in vivo, all data are expressed as the mean ± SD, *P < 0.05, **P < 0.01; relative to the control group or sham group