Table 7.
Fluorescence minus one for murine fluorescence active cell sorting of bone stromal cells.
| Antigen | FITC | PE | PerCP-Cy5.5 | APC |
|---|---|---|---|---|
| MESENCHYMAL STEM CELLS | ||||
| Sca1 (Ly6A/E) FMO | --- | CD146 | CD90 | CD29 |
| CD146 FMO | Sca1 | --- | CD90 | CD29 |
| CD90 FMO | Sca1 | CD146 | --- | CD29 |
| CD29 FMO | Sca1 | CD146 | CD90 | --- |
| MACROPHAGE/ OSTEOCLAST | ||||
| CD11b FMO | --- | CD265 | CD68 | CD115 |
| CD265 (RANK) FMO | CD11b | --- | CD68 | CD115 |
| CD68 FMO | CD11b | CD265 | --- | CD115 |
| CD115 FMO | CD11b | CD265 | CD68 | --- |
| OSTEOBLAST | ||||
| Alkaline Phosphatase FMO | --- | --- | CD90 | --- |
| CD90 FMO | --- | --- | --- | AlkPhos |
| OSTEOCYTE | ||||
| SPARC FMO | --- | GP38 | --- | --- |
| Podoplanin GP38) FMO | --- | --- | --- | SPARC |
| HEMATOPOIETIC STEM CELLS | ||||
| CD34 FMO | Sca1 | --- | --- | --- |
| Sca1 (Ly6A/E) FMO | --- | CD34 | --- | --- |
Fluorescence minus one (FMO) control panel was conducted to identify fluorochrome bleed over with cell-specific antibody mixes. Results indicated no fluorochrome bleed over into the FMO channel. AlkPhos = Alkaline Phosphatase; SPARC = Secreted Protein Acidic and Rich in Cysteine