Table 9.
Isotype control murine antibody staining.
| Isotype Control | Company | Catalog Number | RRID |
|---|---|---|---|
| MESENCHYMAL STEM CELL | |||
| FITC Rat IgG2a, k | BioLegend | 400506 | AB_273619 |
| PE Rat IgG2a, k | BioLegend | 400508 | AB_326530 |
| APC Hamster IgG | BioLegend | 402012 | AB_2814701 |
| PerCP-Cy5.5 Rat IgG2b, k | BioLegend | 400632 | AB_893677 |
| MACROPHAGE/ OSTEOCLAST | |||
| FITC Rat IgG2b, k | BioLegend | 400634 | AB_893662 |
| APC Rat IgG2a, k | BioLegend | 400512 | AB_2814702 |
| PerCP-Cy5.5 Rat IgG2b, k | BioLegend | 400632 | AB_893677 |
| PE Rat IgG2a, k | BioLegend | 400508 | AB_326530 |
| OSTEOBLAST | |||
| APC Rat IgG1, k | BD Biosciences | 555751 | AB_398613 |
| PerCP-Cy5.5 Rat IgG2b, k | BioLegend | 400632 | AB_893677 |
| OSTEOCYTE | |||
| PE Hamster IgG | BioLegend | 400923 | AB_2814703 |
| APC Rat IgG2b, k | BioLegend | 400612 | AB_326556 |
| HEMATOPOIETIC STEM CELL | |||
| PE Rat IgG2a, k | BioLegend | 400508 | AB_326530 |
| FITC Rat IgG2a, k | BioLegend | 400506 | AB_273619 |
Isotype control mixes were utilized as a negative control to determine the contribution of non-specific background to staining and correct for non-specific binding. Results indicated no non-specific binding for cell identification schema.