Fig. 2.

Sialoglycoprotein biosynthetic gene programs are found in cancer cells and elevated in aggressive breast cancers. A. UMAP projection of all cells from scRNAseq data of 34 breast tumors [20]. B. Diagram of sialylglycoprotein biosynthetic pathway. C. Plot of percent sialic acid biosynthesis module-positive (CMAS and NANS) by cell type for each patient. D. Dot plot showing expression of all sialoglycoprotein biosynthetic genes in epithelial cells extracted from normal breast, and HR+, HER2+, and TN tumors. E. Representative images of multicolor IF of CMAS and CK. Scale bar is 100 μm. F. Quantification of CMAS staining in CK-positive cells. For each subtype n = 2–3 patient samples were quantified. The mean is plotted and the error bars represent 1 standard deviation.