Fig. 4.

Mical inhibition upon treatment with EGCG. a (left panel) Virgin female flies bearing the UAS-Mical transgene were crossed with elav^C155-GAL4 males in a medium supplemented with the indicated concentrations of EGCG. Bars represent the mean ratio number of their progeny female versus male ± SEM. Stars indicate significantly altered viability ratios compared to untreated flies (right panel). Virgin female flies carrying the htau^0N4R transgene were crossed with elav^C155-GAL4 males in a medium supplemented or not with 1.5 mM EGCG. w¹¹¹⁸ females were crossed with elav^C155-GAL4 males and the ratio of their progeny female versus male was considered as control progeny without (CN) or with EGCG (CN+). Star indicates significant difference from CN. b Representative Western blot of aqueous soluble and insoluble fractions generated from adult heads following panneuronal expression of Tau in the absence or presence of 1.5 mM EGCG. Star indicates significantly increased solubility of Tau upon treatment with EGCG. c Pellet (p) and supernatant (s) fractions of endogenous microtubules (MT), preformed bovine microtubules (MTexo) and phalloidin-bound F-Actin (Actin) from Tau expressing flies in the absence or presence of 1.5 mM EGCG have been probed for Tau, Tubulin and Actin respectively. Stars indicate significantly altered levels of Tau co-precipitated with microtubules and Actin upon treatment with 1.5 mM EGCG compared to untreated Tau flies