Fig. 5.

The effects of Mical on Tau are mediated by oxidation of Cys322. a Virgin female flies carrying the indicated Tau transgenes were crossed with elav^C155-GAL4 (light grey bar) or elav^C155-GAL4;UAS-Mical/CyO males in a medium supplemented (blue bar) or not (black bar) with 10 μM Methylene Blue (MB). Controls include w¹¹¹⁸ females crossed with elav^C155-GAL4 males (light grey bar) or elav^C155-GAL4;UAS-Mical/CyO in normal food (black bar) or with MB (blue bar). Bars represent the mean number of non-balancer-bearing progeny females over males ± SEM of the indicated genotypes. Stars indicate significant difference between black and blue bars. b Response of flies expressing the indicated transgenes upon treatment with paraquat for 28 h. Stars indicate significant difference from the transgene without Mical overexpression. Control flies are elav^C155-GAL4/+;Ras2-GAL4/+ (grey bar) and Mical are flies that overexpress Mical under the panneuronal double driver (black bar). c Representative Western blots of aqueous soluble and insoluble fractions of the indicated Tau transgenes alone or upon co-overexpression with Mical. Stars indicate significantly reduced solubility from the transgene without Mical overexpression. d Memory performance of animals expressing panneuronally the indicated Tau transgenes (dark grey bar), compared with the same transgene upon co-expression with Mical (black bars). Stars indicate significant differences from the transgene without Mical overexpression. Control flies (light grey bars) are elav^C155-GAL4/+;Ras2-GAL4/+ flies (CN) and flies that overexpress Mical under the panneuronal double driver. e The normalized peak areas were calculated for each of the two variants of the cysteine containing peptide ³²²CGSLGNIHHKPGGGQVEVK and their ratio (NEM versus carbamidomethyl) is shown. The bars represent the mean ± SEM ratio from three biological and two technical replicas. Star indicates significant difference between the two groups