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. 2022 Apr 5;20:154. doi: 10.1186/s12967-022-03305-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — The characterization of circ_0036412 in HCC cells. A GSE113510 dataset was adopted to analyze the circRNAs differentially expressed in HCC tissues compared with normal tissues. B The expressions of circ_0036412, circ_0036411 and circ_0008092 were detected by qRT-PCR in THLE-2, Hep 3B, HuH-7 and Li-7 cells (One-way ANOVA, Tukey). C The circular structure of circ_0036412 was shown. D The expressions of circ_0036412 and ETFA mRNA were detected by qRT-PCR in THLE-2 cells after the treatment of RNase R (Student’s t test). E The expressions of circ_0036412 and ETFA mRNA were detected by qRT-PCR in ActD-treated THLE-2 cells (Student’s t test). F PCR-agarose gel electrophoresis in THLE-2 cells verified the circular structure of circ_0036412. **P < 0.01