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. 2022 Feb 6;9(10):2105466. doi: 10.1002/advs.202105466

Figure 5.

Figure 5

Localization of PBNPs in NPCs. A) Fitc‐PBNPs was treated to NP cells with increasing time and colocalization of Rad5 with Fitc‐PBNPs was detected by Confocal. Statistical chart shows IOD/area of red and green fluorescence. B) TEM images of intracellular mitochondria in NP cells, after H2O2 (0.6 × 10−3%) stimulation and PBNPs (2 µg mL−1) treatment. Statistical chart shows length of mitochondria and width of mitochondria crista. C) NP cells, after H2O2 (0.6 × 10−3%) stimulation and Fitc‐PBNPs (2 µg mL−1) treatment was strained with mito‐Tracker. Statistical chart shows IOD/area of red and green fluorescence. D) After H2O2 (0.6 × 10−3%) stimulation and Fitc‐PBNPs (2 µg mL−1) treatment, colocalization of SOD1 with Fitc‐PBNPs was detected by Confocal. Statistical chart shows IOD/area of red and green fluorescence. The data were presented using mean ± S.D. * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001. The experiments above were repeated six times.