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. 2022 Apr 4;13:143. doi: 10.1186/s13287-022-02816-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — Effects of the MNPs + PCL/Col I and PCL/Col I scaffolds on the osteogenic differentiation of ADSCs seeding on scaffolds. a–c Gene expressions of COL1, OCN and Runx2 by qRT-PCR. d–h Protein level of Col I, OCN, Runx2, BMP2, and ON proteins by Western blot analysis. Both of assays performed on seeded ADSCs at 21 days of incubation in osteo and osteo cues-free media conditions. Data are the representative of three independent experiments. One-Way ANOVA with Tukey post hoc analysis. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001. Negative control (ADSCs alone whiteout osteo media); Positive control (Osteoblast cells)