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. Author manuscript; available in PMC: 2022 Apr 5.
Published in final edited form as: Cancer Cell. 2021 Jul 21;39(8):1115–1134.e12. doi: 10.1016/j.ccell.2021.06.016

Figure 7. Enhancer usage dictates opposite YAP/TEAD effects.

Figure 7.

(A) TEAD1/YAP coimmunoprecipitation in YAPon and YAP-transduced YAPoff cells.

(B) TEAD1 deletion prevents YAP-induced cytostasis. Mean ± SD, n = 4.

(C) Ectopic TEAD3 or TEAD4 enhances YAP-meditated cytostasis. Mean ± SD, n = 3.

(D) TEAD4-VP64 impedes YAPoff cell line growth (n = 3, p < 0.001).

(E) Hierarchical clustering of the top 1,000 TEAD4 ChIP-seq peaks from each YAPon or YAP-transduced YAPoff line.

(F) k-Means clustering of TEAD4 peaks defines 16 enhancer groups.

(G) Unsupervised hierarchical clustering of enriched TF motifs in the 16 TEAD4-enhancer groups. Numbers: motif rank.

(H) Hierarchical clustering of co-bound TFs in the 16 TEAD enhancer groups.

See also Figures S6 and S7 and Table S7.