Figure 4.

Spn-colonized recipient ferrets are susceptible to H1N1pdm09 infection but not severe disease. (A) Schematic representation of cage setup, where recipients were inoculated with 10⁷ CFUs of Spn (D39) 48 h prior (N = 4) to exposure. Donors (N = 4) were infected with 10⁶ TCID₅₀ H1N1pdm09 (A/CA/07/2009) and 24 h later recipients were placed in the adjacent cage and exposed for 2 days. (B) H1N1pdm09 titers from donor and recipient animals. Limit of detection is denoted by a dashed line. (C) Bacterial loads from recipients in panel (B) are indicated by each symbol, which represents an individual animal. (D) Percent number of H1N1pdm09/Spn D39 coinfected recipients from Figure S1D (Supporting Information), and Fig. 2D/2E and 2G/2H (N = 3) and Spn-colonized recipients infected with H1N1pdm09 from panels (B) and (C) (N = 4) displaying each symptom over the course of the experiment.