Figure 2.

M-MDSCs from HFD-fed mice showed stronger osteoclastogenic differentiation potential. (A) Representative TRAP stain in which M-MDSCs from LFD/HFD-fed mice were cultured in osteoclastogenic differentiated medium. (B) Osteoclast cellular endpoints were performed on TRAP-stained LFD/HFD-fed mice. Unpaired t test. Data are presented as mean ± SEM. *P < 0.05. ****P < 0.0001. HFD, high-fat diet; LFD, low-fat diet; M-MDSC, monocytic myeloid-derived suppressor cell; OC, osteoclast; TRAP, tartrate-resistant acid phosphate.