Figure 1:

Dynamic nuclear polarization enhances NMR signals in cellular environments. A) One dimensional ¹³C{1H} CP spectra both with (blue) and without DNP enhancement (red). DNP NMR results in large signal enhancements (ε) for samples of per-deuterated cellular lysates in a 60:30:10 mixtures of d₈-glycerol:D₂O:H₂O and 10 mM AMUPol at 600 MHz/385 GHz with ω/2π = 12.5 kHz and a sample temperature of 100 K. Spectroscopic selection for the small amount of uniformly isotopically labeled exogenous prepared protein that was added to the cellular lysate mixture reveals that the amyloid cores of this protein have similar conformations (purple line), the intrinsically disordered region (red line) has a very different chemical environment in (B) purified samples than it does in (C) in biological environments.