Materials
| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| ACD HybEZII Hybridization system (110V) with ACD EZ-Batch Slides system | ACD | 321710 | Hybridization oven |
| CAT Hematoxylin | Biocare medical | CATHE-GAL | colorstain |
| Clear-Mount | ELECTRON MICROSCOPY SCIENCES | 17985-15 | mounting reagent for red chromogen |
| Immpact DAB Peroxidase Kit | Vector | SK-4105 | Used to reveal HRP - DAB (Brown) to replace the DAB coming in the ACD kit |
| lithium carbonate | Fisher chemical | L119-500 | bluing solution |
| paraformaldehyde | ELECTRON MICROSCOPY SCIENCES | 15714-S | for tissue fixation (4%) |
| PBS | life technology | 14190-136 | |
| Permount Mounting Medium | ThermoFisher Scientific | SP15-100 | mounting regaent for brown chromogen |
| Prolong Gold | ThermoFisher Scientific | P36930 | mounting regaent for fluorescence |
| ribonucleases A | ThermoFisher Scientific | 12091039 | for RNAse treatment in DNAscope protocol |
| ribonucleases T1 | Roche | R1003 | for RNAse treatment in DNAscope protocol |
| RNAscope 2.5, 2-plex detection reagent | ACD | 322430 | Brown and red kit chromogen detection |
| RNAscope Target Retrieval Reagents | ACD | 322000 | retrieval buffer |
| SuperFrost Plus Glass Slides | ThermoFisher Scientific | 12-550-17 | |
| TBS | BOSTON BIOPRODUCTS | BM-301-4L | for washes |
| TSA Plus Fluorescence palette kit (Cy3, Cy5, TMR, Fluorescein) | Perkin elmer | NEL760001KT | HRP Fluorescence detection |
| Tween 20 | SIGMA | P1379-1L | for washes |
| XYLENE 20LT | ThermoFisher Scientific | AC422680200 |