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. 2021 Dec 14;73(7):2206–2221. doi: 10.1093/jxb/erab550

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© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Wss, Psl, and α-glucan production are stimulated by low temperature. (A) Effect of temperature on Pst colony morphology on CR-supplemented media. Wild-type (WT) Pst and Pst wspR19 grown on KB and SFM at 28 °C and 8 °C. (B) qRT–PCR analysis of polysaccharide genes in WT Pst grown on KB agar at 28 °C (black) and 8 °C (grey). (C) Colony morphology of the WT, Δpsl, and Δwss on KB agar after 5 d of cold treatment at 8 °C. Strains with high cyclic-di-GMP (wspR19) are shown. (D) qRT–PCR analysis of polysaccharide genes in Pst wspR19 grown on KB agar at 28 °C (black) and 8 °C (grey). Values represented are means ±SD with n=2 (three technical replicates each). Significance was determined by Student’s t-test (∗∗P<0.005 and ∗∗∗∗P<0.0001). (A and C) Similar results were obtained in two independent experiments and a representative image is shown.