Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 14;73(7):2206–2221. doi: 10.1093/jxb/erab550

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 4. — α-Glucan and alginate biosynthesis are stimulated by global carbon storage regulators and surface association. (A) ¹H-NMR spectra for Pst wild type (WT; blue), ΔtreS (pink), ΔtreY/Z (green), and a double mutant ΔtreS ΔtreY/Z (purple). Peaks corresponding to key metabolites are labelled: maltose 1-phosphate (M1P), α-glucan (α-1,4), α-1,1-probable terminal linkage of maltooligosyltrehalose (trehalose). (B) qRT–PCR analysis of polysaccharide genes in WT Pst grown on KB agar (solid) versus KB liquid (liquid). Values represented are means ±SD with n=2 (three technical replicates each). Significance was determined by Student’s t-test (∗∗∗∗P<0.0001. (C) ¹H-NMR spectra for the Pst PglgA-lacZ reporter strain and selected glgA regulatory Tn mutants. Peaks corresponding to key metabolites are labelled as in (A).