Table 2.

Primers used for glutamine synthetase (GS) gene cloning and expression analysis in Eleusine indica

Primers	Sequence (5ʹ–3ʹ)
GS gene cDNA cloning
EiGS1-1-F	ATGGCCTCCCTCACCGACCTC
EiGS1-1-R	CACCACTAAATAGATGGACGAG
EiGS1-2-F	TGCTTTGGCTTGTGAGATATGG
EiGS1-2-R	TGCACAGATCGTCACTCCGGC
EiGS1-3-F	TGCGACGCACAATTCACTCGC
EiGS1-3-R	GCTCGCACAAACGCATACCC
EiGS2-F	AGGATGTGGGGCGCCAGGAG
EiGS2-R	AATCACAAGTTCCAGACCGACA
GS gene expression analyses (RT-qPCR)
qGS1-1-F	AGTCATTCGGGCGTGACATT
qGS1-1-R	AATGTAGCGAGCAACCCACA
qGS1-2-F	GCCGACATCAACACCTTC
qGS1-2-R	GGTAATCAGTTTCCCTTCCA
qGS1-3-F	AGCCCAGCCACTCCAATG
qGS1-3-R	GCGAAGTCCCAATAATACAAA
qGS2-F	GGTGTGGTGCTTACCCTTGA
qGS2-R	TTCACGCATGGTCTTTGTGC
eIF-4-F (reference gene)	CCTACCAAAACGACCACTACGAC
eIF-4-R (reference gene)	ATCACCACCGACCTCCTTGCTC
pOX-GS recombinant vector construction and detection
pOX-GS1-F	TGTTACTTCTGCAGGGTACCATGGCCTCCCTCACCGACCTC
pOX-GS1-R	CGGATCCATAACGCGTTCAAGGCTTCCAGATGATGGTGGTC
Hyg-F	GACCTGCCTGAAACCGAACTG
Hyg-R	CCCAAGCTGCATCATCGAAA
Transgene copy detection
TansGS1-F	AGAACGGCAAGGGCTACTTC
TansGS1-R	CCAGATGATGGTGGTCTCGG
SPS-F	TTGCGCCTGAACGGATAT
SPS-R	CGGTTGATCTTTTCGGGATG
Yeast positive cloning test
Forward primer	CGTAGAATTCATGCACCATCACCATCACCATGCCTCCCTCACC
Reverse primer	TGTCTAAGGCGAATTAATTCGCGGCCGCTCAAGGCTTCCAG