Fig. 3.

In vivo visualization of α-galactosidase activity in germinating seeds of O. minor with 5-bromo-4-chloro-3-indolyl-α-d-galactopyranoside (X-α-Gal). The seeds at (A) 3 d after rac-GR24 treatment (DAT) or (B) 5 DAT were treated with X-α-Gal solution (40 µg ml⁻¹). The endosperm and seed coat were separated manually from the embryo with emerging radicles at (C) 3 DAT or (D) 5 DAT, and treated with X-α-Gal solution. Arrowheads indicate the pigmentation of X-α-Gal. Scale bars=100 µm.