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Depletion of CD4+ T cells, CD8+ T cells and NK cells impaired the antitumor effect of CXCL13. BALB/c mice were injected on the right dorsal flank with 2 × 105 4T1, 4T1-PCDH, and 4T1-CXCL13-2 cells. On days –1, 1, 7, and 14, mouse CD4 antibody, mouse CD8a antibody or mouse NK antibody was injected. Tumors were measured every 3 days starting on day 7; tumor volumes were calculated as in Fig. 6. Bars, means ± SD (*P < 0.05, **P < 0.01, ****P < 0.0001, n = 3).
