Figure 6. Iron supplementation rescues mitochondrial function.

• A
  Mitochondrial iron quantified by ICP‐MS in quadriceps of C26 tumor‐bearing mice after FeCM supplementation (n = 7–8).
• B
  Aconitase activity of quadriceps lysates normalized to protein content (n = 3–7).
• C
  Succinate Dehydrogenase activity staining of gastrocnemius transversal sections and resulting intensity quantification (n = 3–7).
• D
  Mitochondrial ATP content determined by luminescence assay in quadriceps (n = 3–9).
• E
  Representative Western blot and densitometric quantification of phospho‐AMPK and total AMPK (stripped and re‐blotted) in the gastrocnemius of C26 tumor‐bearing mice after iron carboxymaltose supplementation (n = 6).
• F–H
  mRNA levels of ACOT 1 (F), MCD (G), and CPT1B (H) normalized to GAPDH in gastrocnemius (n = 5–10).

Data information: For all data, n represents the number of biological replicates. Statistical significance was calculated by unpaired, two‐tailed Student’s t‐test. Data are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 compared to control and #P < 0.05, ##P < 0.01, ###P < 0.001 compared to C26‐untreated group.

Source data are available online for this figure.