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. 2022 Feb 14;23(4):e52904. doi: 10.15252/embr.202152904

Figure EV2. Biological specificity of 4D7.

Figure EV2

  1. Cytokine‐dependent TF‐1 TpoR cells with an overexpression of WT CALR cultured in the absence of TPO, 10 ng/ml hTPO and 10 or 20 µg/ml 4D7 or 20 µg/ml control IgG antibody for 5 days and the number of trypan blue‐negative cells were counted every 24 h (n = 3 biological replicates with three technical replicates).
  2. Cytokine‐dependent TF‐1 CALRdel52 cells lacking TpoR were cultured in the presence of 2 ng/ml GM‐CSF and 10 or 20 µg/ml 4D7 or 20 µg/ml control IgG antibody (n = 3 biological replicates with three technical replicates).
  3. Cytokine‐dependent TF‐1 CALRdel61 cells lacking TpoR were cultured in the presence of 2 ng/ml GM‐CSF and 10 or 20 µg/ml 4D7 or 20 µg/ml control IgG antibody (n = 3 biological replicates with three technical replicates).
  4. MARIMO cells from which the CALRdel61 mutation was originally amplified were cultured in the presence of 2, 10 or 20 µg/ml 4D7 or 20 µg/ml control IgG antibody (n = 3 biological replicates with three technical replicates).
  5. Cytokine‐independent SET2 cells which harbour the pathogenic JAK2V617F mutation were cultured in the presence of 2, 10 or 20 µg/ml 4D7 or 20 µg/ml control IgG antibody (n = 3 biological replicates with three technical replicates).
  6. Cytokine‐independent TF‐1 PTPN11E76K cells were cultured in the presence of 10 or 20 µg/ml 4D7 or 20 µg/ml control IgG antibody (n = 3 biological replicates with three technical replicates).

Data information: For all panels, bars represent standard error of the mean.