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. 2021 Aug 28;4(4):231–245. doi: 10.1093/pcmedi/pbab023

Figure 3.

Figure 3.

Effects of GLSF GI juice extract on P-glycoprotein activity in K562/DOX cells and NF-κB activity in HEK-293 cells. (A) Flow cytometry analysis of DNR accumulation in cells incubated with DNR, treated with or without PSC833 (10 μM) or GLSF. Representative flow cytometry data are shown. (B) Graphs showing percentage of cells that were positive for DNR in cell samples co-treated with GLSF and DNR in comparison with those co-treated with 10 μM PSC833 and DNR. The data are presented as the mean ± SD from 3–6 independent experiments. *: P < 0.05 compared to the DNR only group. (C) Effects of GLSF on TNF-α-induced NF-κB promoter activity. HEK-293 cells transfected with NF-κB luciferase reporter and Renilla control reporter were treated with vehicle, TNF-α (10 ng/mL), GLSF (0.3, 1.0, 2.0 mg/mL) combined with TNF-α for 5 hours. Data are expressed as mean +/- SEM; n = 3–6. *: P < 0.05 as per one-way ANOVA.