Figure 5. β1 integrin facilitates αSMA⁺ myofibroblast‐mediated wound repair.

• A
  Violin plot of Itgb1 expression levels in fibroblasts from murine wounds. Raw scRNA‐seq data are derived from Haensel et al (2020b), Data ref: Haensel et al (2020a).
• B
  UMAP plots of ACTA2 and ITGB1 expression of fibroblasts in human wounds. Raw scRNA‐data are derived from Davis et al (2020c), Data ref: Davis et al (2020b).
• C
  IF for β1 integrin (red) and αSMA (green) on wound tissue sections at day 17 post‐wounding. White boxes denote zoomed regions depicted in right panels. Scale bar: 20 μm.
• D
  Quantification of the number of αSMA⁺ β1 integrin⁺ cells. WT, N = 3; TK, N = 3 biological replicates. Unpaired t‐test performed comparing WT to TK.
• E
  Wound area measurements in WT and Itgb1^cKO mice. WT, N = 5; Itgb1^cKO, N = 6 biological replicates. Two‐way ANOVA performed comparing WT and Itgb1^cKO mice.
• F–K
  Reepithelization, granulation tissue formation, and vascularization in WT and Itgb1^cKO mice. (F) Representative images of keratin 5 (Krt5, red) stained wounds at day 14 post‐wounding. White boxes denote zoomed regions depicted in right panels. Scale bar, 500 µm. (G) Quantification of epidermal thickness. WT, N = 4; Itgb1^cKO, N = 6 biological replicates. (H) Immunofluorescent staining for CD31 (red) and nuclei (blue) on wound tissue sections at day 14 post‐wounding. White boxes denote zoomed regions depicted in right panels. Scale bar, 20 μm. (I) Quantification of CD31⁺ area per visual field normalized to WT mice. WT, N = 6; Itgb1^cKO, N = 6 biological replicates. (J) Polarized light images of picrosirius red stained wound tissues at day 14 post‐wounding. Red: collagen fibers. White boxes denote zoomed regions depicted in right panels. Scale bar, 50 µm. (K) Quantification of collagen fiber percent area normalized to the average area in WT controls. WT, N = 6; Itgb1^cKO, N = 6 biological replicates.
• L
  Quantification of collagen fiber width normalized to the average width in WT controls. WT, N = 6; Itgb1^cKO, N = 6 biological replicates.

Data information: Data are reported as mean ± s.e.m. for (D, E, G, I, K, and L). Data are reported as median (open circle) with interquartile range indicated with bar for violin plots (A). One sample t‐test performed comparing values to WT average for (G, I, K, and L). Exact P‐values are reported.

Source data are available online for this figure.