Figure 5. Differential genetic requirements common to rewired libraries.

1. Volcano plot comparing the number of transposon insertions per gene of all 24 rewired libraries and two wild‐type (wt) libraries. Genes that are significantly (P‐value < 0.05) required (red, log₂[fold change] < −1.5; blue, log₂[fold change] < −1) or dispensable (green, log₂[fold change > 2]) in the rewired libraries are highlighted. Genes depicted in grey are essential due to differential growth requirements in the preparation of the two wt libraries compared to the rewired strains, or correspond to genes deleted in the rewired strains.
2. Requirement for mitochondrial lipid transport and biosynthesis genes in rewired libraries. Left panel, schematic depicting PE and cardiolipin (CL) synthesis in mitochondria. The inner mitochondrial putative lipid transporter Mdm31 is depicted in yellow. Gray arrows indicate the need for lipid transport from the ER or other organelles to mitochondria and transport between the MOM and MIM. Right panel, transposon numbers (normalized to wt libraries) for the indicated (set of) libraries for MDM31, PGS1, and CRD1.

Source data are available online for this figure.