Figure 2. Galactose‐grown cells present with stronger HyPer7 responses upon reoxygenation after hypoxia.

• A–D
  Response of HyPer7 probes targeted to indicated compartments to reoxygenation after hypoxia (red curve and data points) or to continued hypoxia (black line and data points). HEK293 cells were grown either with glucose or galactose as carbon source. Solid line represents mean, points colored in the lighter version of the respective color are the corresponding ratios measured in individual cells.
• E
  Model. Cells grown on galactose‐containing medium exhibit increased production of H₂O₂ and thus also increased detection of cytosolic H₂O₂ indicating H₂O₂ gradients of different steepness around mitochondria.

Data information: The numbers of cells per experiment for each dataset can be found in Appendix Table S6. As most of the data were not normal distributed, instead of a t‐test, a Wilcoxon/Mann–Whitney‐U‐test was performed and samples were compared in pairs. ***P ≤ 0.001.

Source data are available online for this figure.