Figure 4. Growth in the presence of different carbon sources impacts compartmental H₂O₂ handling.

1. Response of HyPer7 probes targeted to indicated compartments to treatment with increasing amounts of exogenous H₂O₂ (2, 4, and 8 µM of H₂O₂; 20 mM of DTT). HEK293 cells were grown either with glucose (black curve and data points) or galactose (red curve and data points) as carbon source. The numbers of cells per experiment for each dataset can be found in Appendix Table S6. As most of the data were not normal distributed, instead of a t‐test, a Wilcoxon/Mann–Whitney‐U‐test was performed and samples were compared in pairs. ***P ≤ 0.001.
2. Model. (Mitochondrial) H₂O₂ is more efficiently handled in galactose‐grown cells in mitochondria and cytosol.

Source data are available online for this figure.