Figure 6. Increased levels of cytosolic thioredoxin reductase in HeLa cells prevent mitochondrial H₂O₂ release to the cytosol.

1. TXNRD1 levels in HeLa and HEK293 cells. Data are from Geiger et al (2012).
2. Response of HyPer7 probes targeted to indicated compartments to incubation with antimycin A (AntA, red curve, and data points) or ethanol as control (black curve and data points). HeLa cells were grown with galactose as carbon source. Solid line represents average, points colored in the lighter version of the respective color are the corresponding ratios measured in individual cells.
3. Response of HyPer7 probes targeted to indicated compartments to treatment of mtDAO‐expressing HEK293 (black curve and data points; cell generated with the piggyBAC system) or HeLa (red curve and data points; cell generated with the piggyBAC system) cells with 8 mM of D‐Ala. The cells were grown with glucose as carbon source. Solid line represents average, points colored in the lighter version of the respective color are the corresponding ratios measured in individual cells.
4. Model. Cells‐ and metabolism‐specific empowerment of the thioredoxin reductase system controlling H₂O₂ concentrations following mitochondrial release.

Data information: The numbers of cells per experiment for each dataset can be found in Appendix Table S6. As most of the data were not normal distributed, instead of a t‐test, a Wilcoxon/Mann–Whitney‐U‐test was performed and samples were compared in pairs. ***P ≤ 0.001.

Source data are available online for this figure.