Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Recombinant DNA reagent |
Plasmid: pRK172-0N4R | PMID:2124967 | NCBI ReferenceSequence:NM_005910.5 | All constructs were derived from this plasmid |
| Strain, strain background (Escherichia coli) |
BL21(DE3) | Agilent | 200,131 | Chemically competent cells |
| Software, algorithm |
RELION | PMID:30412051 | RRID:SCR_016274 | RELION 4.0Helical reconstruction |
| Software, algorithm |
Coot | PMID:20383002 | RRID:SCR_014222 | Model building |
| Software, algorithm |
ISOLDE | PMID:20383002 | Model refinement |