Table 2. Data acquisition details for the different microscopes.
| Microscope | LMB Krios G1 | LMB Krios G2 | TFS Glacios | TFS Krios G4 |
|---|---|---|---|---|
| Magnification | 105,000 | 96,000 | 165,000 | 165,000 |
| Camera | K2*/K3 | Falcon 4 | Falcon 4 | Falcon 4 |
| Energy filter (eV) | 20 | NA | 10 | 10 |
| Voltage (kV) | 300 | 300 | 200 | 300 |
| Electron exposure (e–/Å2) | 40 | 30/40 | 40 | 40 |
| Defocus range (μm) | 1.5–3 | 1.2–2.5 | 0.6–1.2 | 0.6–1.2 |
| Pixel size (Å) | 0.85/1.145* | 0.824 | 0.672 | 0.727 |
LMB: Laboratory of Molecular Biology.
*
LMB Krios G1 initially had a K2 camera, which was later replaced by a K3 camera. Only condition 1 a (as defined in Table 1) was collected on the K2 camera. The pixel size on the K2 camera was 1.145 Å.