Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6NJcl | Clea Japan | ||
| Strain, strain background (M. musculus) | C57BL/6-Ly5.1 | Riken Bioresource Center | RRID:IMSR_RBRC00144 | |
| Strain, strain background (Sendai virus) | Sendai virus KOSM302L | Dr. Mahito Nakanishi (TOKIWA-Bio Inc) | ||
| Cell line (M. musculus) | L3-1, L7-1 to -8, L11-1 to -4, L15-1 to -3, L19-1 to -6 | This study | Murine MAIT cell-derived iPSCs | |
| Cell line (M. musculus) | Mouse embryonic fibroblast (MEF) | Oriental Yeast | Cat# KBL9284600 | |
| Cell line (M. musculus) | OP9 -DLL1 cell | Dr. Hiroshi Kawamoto (Kyoto University) | ||
| Cell line (M. musculus) | B16F10 cell | Dr. Tsukasa Seya (Hokkaido University) | RRID:CVCL_0159 | |
| Cell line (M. musculus) | Lewis lung carcinoma (LLC) | Riken Bioresource Center | Cat# RCB0558; RRID:CVCL_4358 | |
| Cell line (M. musculus) | EL4 | Riken Bioresource Center | Cat# RCB1641; RRID:CVCL_0255 | |
| Cell line (M. musculus) | RL-♂1 (Gloria) | Riken Bioresource Center | Cat# RCB2784; RRID:CVCL_C832 | |
| Cell line (M. musculus) | Yac-1 | Riken Bioresource Center | Cat# RCB1165; RRID:CVCL_2244 | |
| Cell line (M. musculus) | CH27 | Dr. X. Wang (Washington University, MO) | ||
| Cell line (M. musculus) | CH27/mMR1 | Dr. X. Wang (Washington University, MO) | ||
| Cell line (M. musculus) | WT3 | Dr. X. Wang (Washington University, MO) | ||
| Cell line (M. musculus) | WT3/mMR1 | Dr. X. Wang (Washington University, MO) | ||
| Antibody | Anti-mouse CD4 (RM4-4), PerCP/Cy5.5 (rat monoclonal) | BioLegend | Cat# 116012; RRID:AB_2563023 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD8α (53–6.7), APC/Cy7 (rat monoclonal) | BioLegend | Cat# 100714; RRID:AB_312753 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD25 (PC61), APC (rat monoclonal) | BioLegend | Cat# 102012; RRID:AB_312861 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD25 (PC61), BV421 (rat monoclonal) | BioLegend | Cat# 102043; RRID:AB_2562611 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD25 (PC61), BV711 (rat monoclonal) | BD Biosciences | Cat# 740652; RRID:AB_2740341 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD25 (PC61), PE (rat monoclonal) | BioLegend | Cat# 102007; RRID:AB_312856 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse/human CD44 (IM7), FITC (rat monoclonal) | BioLegend | Cat# 103006; RRID:AB_312957 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD45.1 (Ly5.1) (A20), BV605 (mouse monoclonal) | BioLegend | Cat# 110738; RRID:AB_2562565 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD45.2 (Ly5.2) (104), PE (mouse monoclonal) | BioLegend | Cat# 109807; RRID:AB_313444 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse/human CD45R/B220 (RA3-6B2), BV421 (rat monoclonal) | BioLegend | Cat# 103239; RRID:AB_10933424 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse/human CD45R/B220 (RA3-6B2), PE (rat monoclonal) | BioLegend | Cat# 103207; RRID:AB_312992 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD49b (DX5), PE (rat monoclonal) | BioLegend | Cat# 108907; RRID:AB_313414 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD69 (H1.2F3), APC/Cy7 (Armenian hamster monoclonal) | BioLegend | Cat# 104525; RRID:AB_10683447 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD69 (H1.2F3), PE (Armenian hamster monoclonal) | BD Biosciences | Cat# 553237; RRID:AB_394726 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD69 (H1.2F3), PE/Cy7 (Armenian hamster monoclonal) | BioLegend | Cat# 104511; RRID:AB_493565 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD107a (1D4B), BV421 (rat monoclonal) | BioLegend | Cat# 121618; RRID:AB_2749905 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD127 (IL-7Rα) (A7R34), PerCP/Cy5.5 (rat monoclonal) | BioLegend | Cat# 135021; RRID:AB_1937274 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD186 (CXCR6) (SAO51D1), PE (rat monoclonal) | BioLegend | Cat# 151103; RRID:AB_2566545 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD218a (IL-18Rα) (P3TUNYA), eFluor450 (rat monoclonal) | Thermo Fisher Scientific | Cat# 48-5183-80; RRID:AB_2574068 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse CD218a (IL-18Rα) (REA947), PE (mouse monoclonal) | Miltenyi Biotech | Cat# 130-115-704; RRID:AB_2727158 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse F4/80 (BΜ8), BV421 (rat monoclonal) | BioLegend | Cat# 123131; RRID:AB_10901171 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse F4/80 (BΜ8), PE (rat monoclonal) | BioLegend | Cat# 123109; RRID:AB_893498 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-human/mouse/rat MR1 (26.5), APC (mouse monoclonal) | BioLegend | Cat# 361107; RRID:AB_2563193 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse NK1.1 (PK136), BV510 (mouse monoclonal) | BD Biosciences | Cat# 563096; RRID:AB_2738002 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse NK1.1 (PK136), FITC (mouse monoclonal) | BioLegend | Cat# 108706; RRID:AB_313393 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse PLZF (9E12), PE (Armenian hamster monoclonal) | BioLegend | Cat# 145803; RRID:AB_2561966 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse RORγt (Q31-378), BV421 (mouse monoclonal) | BD Biosciences | Cat# 562894; RRID:AB_2687545 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse TCR Vβ6 (RR4-7), PE (mouse monoclonal) | BioLegend | Cat# 140003; RRID:AB_10640727 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse TCR Vβ8.1, 8.2 (MR5-2), PE (mouse monoclonal) | BioLegend | Cat# 140103; RRID:AB_10641144 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse TCRβ (H57-597), BV605 (Armenian hamster monoclonal) | BioLegend | Cat# 109241; RRID:AB_2629563 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-mouse TCRβ (H57-597), PE/Cy7 (Armenian hamster monoclonal) | BioLegend | Cat# 109222; RRID:AB_893625 | Flow cytometry (1:100 in 50 µl reaction) |
| Antibody | Anti-human/mouse/rat MR1 (26.5), purified (mouse monoclonal) | BioLegend | Cat# 361110; RRID:AB_2801000 | Blocking assay (10 µg/ml) |
| Antibody | Mouse IgG2a, k isotype control (MOPC-173), purified (mouse monoclonal) | BioLegend | Cat# 400264; RRID:AB_11148947 | Blocking assay (10 µg/ml) |
| Antibody | Anti-human/mouse/rat β-actin (2F1-1), purified (mouse monoclonal) | BioLegend | Cat# 643802; RRID:AB_2223199 | Western blotting (1:2000 in 5 ml reaction) |
| Antibody | Anti-human/mouse LAT (11B.12), purified (mouse monoclonal) | Santa Cruz | Cat# sc-53550; RRID:AB_784283 | Western blotting (1:1000 in 5 ml reaction) |
| Antibody | Anti-phosphotyrosine (PY99), purified (mouse monoclonal) | Santa Cruz | Cat# sc-7020; RRID:AB_628123 | Western blotting (1:1000 in 5 ml reaction) |
| Antibody | Anti-mouse/rat Asialo GM1 (rabbit polyclonal) | FUJIFILM Wako | Cat# 014-09801 | In vivo NK depletion (50 µg/mouse) |
| Sequence-based reagent | ADV19 | This study | PCR primer (detection of rearranged TCRVα19-Jα33) | 5′-TCAACTGCACAT ACAGCACCTC-3′ |
| Sequence-based reagent | AJ33 | This study | PCR primer (detection of rearranged TCRVα19-Jα33) | 5′-CATGCATTATTCA GCCAGTGCCTTCT-3′ |
| Sequence-based reagent | TRAV19-F | This study | PCR primer (Southern blot probe synthesis) | 5′-CCTGGACCACATG GAAGCATGGC-3′ |
| Sequence-based reagent | TRAV19-R | This study | PCR primer (Southern blot probe synthesis) | 5′-CCCAGAGCC CCAGATCAAC-3′ |
| Sequence-based reagent | TRBV13 | This study | PCR primer (identification of TCRβ repertoire) | 5′-GTACTGGTAT CGGCAGGAC-3′ |
| Sequence-based reagent | TRBV19 | This study | PCR primer (identification of TCRβ repertoire) | 5′-GGTACCGAC AGGATTCAG-3′ |
| Sequence-based reagent | TRBC-Rev | This study | PCR primer (identification of TCRβ repertoire) | 5′-GGGTAGCCT TTTGTTTGTTTG-3′ |
| Sequence-based reagent | Gapdh-F | This study | PCR primer (semi-quantitative PCR) | 5′-CATCACTGCCAC CCAGAAGACTG-3′ |
| Sequence-based reagent | Gapdh-R | This study | PCR primer (semi-quantitative PCR) | 5′-ATGCCAGTGAGC TTCCCGTTCAG-3′ |
| Sequence-based reagent | Tnf-F | This study | PCR primer (semi-quantitative PCR) | 5′-CCACCACGC TCTTCTGTCTAC-3′ |
| Sequence-based reagent | Tnf-R | This study | PCR primer (semi-quantitative PCR) | 5′-AGGGTCTGG GCCATAGAACT-3′ |
| Sequence-based reagent | Ifng-F | This study | PCR primer (semi-quantitative PCR) | 5′-AAAGAGATAAT CTGGCTCTGC-3′ |
| Sequence-based reagent | Ifng-R | This study | PCR primer (semi-quantitative PCR) | 5′-GCTCTGAGAC AATGAACGCT-3′ |
| Sequence-based reagent | Grza-F | This study | PCR primer (semi-quantitative PCR) | 5′-GGTGGAAAG GACTCCTGCAA-3′ |
| Sequence-based reagent | Grza-R | This study | PCR primer (semi-quantitative PCR) | 5′-GCCTCGCAA AATACCATCACA-3′ |
| Sequence-based reagent | Grzb-F | This study | PCR primer (semi-quantitative PCR) | 5′-ACTCTTGACG CTGGGACCTA-3′ |
| Sequence-based reagent | Grzb-R | This study | PCR primer (semi-quantitative PCR) | 5'-AGTGGGGCT TGACTTCATGT-3′ |
| Sequence-based reagent | Grzk-F | This study | PCR primer (semi-quantitative PCR) | 5′-AAGCTTCGCACT GCTGCAGAACT-3′ |
| Sequence-based reagent | Grzk-R | This study | PCR primer (semi-quantitative PCR) | 5′-TAACAGATCTGG CTTGGTGGTTCC-3′ |
| Sequence-based reagent | Prf1-F | This study | PCR primer (semi-quantitative PCR) | 5'-CTCTCGAAGTG TTGGATACAG-3′ |
| Sequence-based reagent | Prf1-R | This study | PCR primer (semi-quantitative PCR) | 5'-GACACAAACGTG ATTCAAATCC-3' |
| Sequence-based reagent | Fasl-F | This study | PCR primer (semi-quantitative PCR) | 5′-GAAGGAACTGGC AGAACTCCGT-3′ |
| Sequence-based reagent | Fasl-R | This study | PCR primer (semi-quantitative PCR) | 5′-GCCACACTCCT CGGCTCTTTTT-3′ |
| Sequence-based reagent | Tnfsf10-F | This study | PCR primer (semi-quantitative PCR) | 5′-GGAAGACCTCAG AAAGTGGCAG-3′ |
| Sequence-based reagent | Tnfsf10-R | This study | PCR primer (semi-quantitative PCR) | 5′-TTTCCGAGAG GACTCCCAGGAT-3′ |
| Sequence-based reagent | Il6-F | This study | PCR primer (semi-quantitative PCR) | 5′-TACCACTTCACA AGTCGGAGGC-3′ |
| Sequence-based reagent | Il6-R | This study | PCR primer (semi-quantitative PCR) | 5′-CTGCAAGTGCAT CATCGTTGTTC-3′ |
| Sequence-based reagent | Il17a-F | This study | PCR primer (semi-quantitative PCR) | 5′-CAGACTACCTC AACCGTTCCAC-3′ |
| Sequence-based reagent | Il17a-R | This study | PCR primer (semi-quantitative PCR) | 5′-TCCAGCTTTCC CTCCGCATTGA-3′ |
| Sequence-based reagent | Il22-F | This study | PCR primer (semi-quantitative PCR) | 5′-GCTTGAGGTGT CCAACTTCCAG-3′ |
| Sequence-based reagent | Il22-R | This study | PCR primer (semi-quantitative PCR) | 5′-ACTCCTCGGAA CAGTTTCTCCC-3′ |
| Sequence-based reagent | Ccl5-F | This study | PCR primer (semi-quantitative PCR) | 5′-CCTGCTGCTTT GCCTACCTCTC-3′ |
| Sequence-based reagent | Ccl5-R | This study | PCR primer (semi-quantitative PCR) | 5′-ACACACTTGGC GGTTCCTTCGA-3′ |
| Sequence-based reagent | Ccl3-F | This study | PCR primer (semi-quantitative PCR) | 5′-ACTGCCTGCTGC TTCTCCTACA-3′ |
| Sequence-based reagent | Ccl3-R | This study | PCR primer (semi-quantitative PCR) | 5′-ATGACACCTGGC TGGGAGCAAA-3′ |
| Sequence-based reagent | Ccl4-F | This study | PCR primer (semi-quantitative PCR) | 5′-ACCCTCCCACT TCCTGCTGTTT-3′ |
| Sequence-based reagent | Ccl4-R | This study | PCR primer (semi-quantitative PCR) | 5′-CTGTCTGCCTC TTTTGGTCAGG-3′ |
| Sequence-based reagent | Ccl22-F | This study | PCR primer (semi-quantitative PCR) | 5′-GTGGAAGACAG TATCTGCTGCC-3′ |
| Sequence-based reagent | Ccl22-R | This study | PCR primer (semi-quantitative PCR) | 5′-AGGCTTGCGGC AGGATTTTGAG-3′ |
| Peptide, recombinant protein | Mouse MR1 5-OP-RU tetramer, APC-labeled | NIH Tetramer Core Facility | Flow cytometry (1:1,000 in 50 µl reaction) | |
| Peptide, recombinant protein | Mouse MR1 5-OP-RU tetramer, BV421-labeled | NIH Tetramer Core Facility | Flow cytometry (1:1,000 in 50 µl reaction) | |
| Peptide, recombinant protein | Mouse MR1 5-OP-RU tetramer, unlabeled | NIH Tetramer Core Facility | MAIT cell stimulation (1:100 to 1:100,000 in 100 µl reaction) | |
| Peptide, recombinant protein | Mouse MR1 6-FP tetramer, APC-labeled | NIH Tetramer Core Facility | Flow cytometry (1:1,000 in 50 µl reaction) | |
| Peptide, recombinant protein | Mouse MR1 6-FP tetramer, BV421-labeled | NIH Tetramer Core Facility | Flow cytometry (1:1,000 in 50 µl reaction) | |
| Peptide, recombinant protein | Mouse MR1 6-FP tetramer, unlabeled | NIH Tetramer Core Facility | MAIT cell stimulation (1:100 to 1:100,000 in 100 µl reaction) | |
| Commercial assay or kit | LEGENDPlex mouse Th cytokine panel | BioLegend | Cat# 740741 | |
| Commercial assay or kit | LEGENDPlex mouse cytokine panel 2 | BioLegend | Cat# 740134 | |
| Commercial assay or kit | LEGENDPlex mouse proinflammatory chemokine panel | BioLegend | Cat# 740451 | |
| Commercial assay or kit | MojoSort mouse NK cell isolation kit | BioLegend | Cat# 480050 | |
| Commercial assay or kit | Vybrant CFDA SE cell tracer kit | Thermo Fisher Scientific | Cat# V12883 | |
| Commercial assay or kit | Zombie Violet Flexible Viability kit | BioLegend | Cat# 423113 | |
| Chemical compound, drug | 5-Amino-4-D-ribitylaminouracil Dihydrochloride | Toronto Research Chemicals | Cat# A629245 | |
| Software, algorithm | FlowJo software v9 and v10 | BD Biosciences | RRID:SCR_008520 | |
| Software, algorithm | Prism 9 for macOS | GraphPad Software | RRID:SCR_002798 |