Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | BL21(DE3)pLysS | Promega | L1195 | – |
| Cell line (human) | Expi293F | Thermo Fisher Scientific | A14527 | – |
| Cell line (human) | PaTu8902 | https://doi.org/10.7554/eLife.45313.001 | – | – |
| Cell line (human) | KP-4 | https://doi.org/10.7554/eLife.45313.001 | – | – |
| Cell line (human) | RWPE-1 | https://doi.org/10.1073/pnas.2018861118 | – | Control and Myc overexpression lines |
| Antibody | Anti-HisTag-650 (mouse monoclonal) | Invitrogen | MA1-21315-D650 | 1:100, flow cytometry, immunocytochemistry |
| Antibody | Biotin-conjugated anti-HRP (rabbit polyclonal) | Rockland | 200-4638-0100 | 1:100, flow cytometry, immunocytochemistry |
| Antibody | Anti-ANPEP (sheep polyclonal) | R&D Systems | AF3815 | 1:1000 |
| Antibody | Anti-Vimentin (rabbit monoclonal) | Cell Signaling Technology | 5741S | 1:1000 |
| Antibody | Anti-FN1 (rabbit polyclonal) | Abcam | ab2413 | 1:1,000 |
| Antibody | Anti-HLA-B (rabbit polyclonal) | ProteinTech | 17260-1-AP | 1:1,000 |
| Antibody | Anti-ITIH4 (rabbit polyclonal) | Atlas antibodies | HPA003948 | 1:1,000 |
| Antibody | Anti-MFGE8 (rabbit polyclonal) | Thermo Fisher Scientific | PA5-82036 | 1:1,000 |
| Antibody | Anti-IGSF8 (goat polyclonal) | R&D Systems | AF3117-SP | 1:1,000 |
| Antibody | Goat Anti-Rabbit HRP (goat polyclonal) | Thermo Fisher Scientific | 31460 | 1:10,000 |
| Antibody | Rabbit Anti-Sheep HRP (rabbit polyclonal) | Thermo Fisher Scientific | 31480 | 1:10,000 |
| Recombinant DNA reagent | APEX2 | Twist Biosciences | – | – |
| Sequence-based reagent | DBCO-DNA (Conjugated to APEX2) | IDT | – | – |
| Sequence-based reagent | Lipid DNA (Anchor and Co-Anchor) | https://doi.org/10.1038/s41592-019-0433-8 | – | – |
| Peptide, recombinant protein | HRP, Peroxidase | Thermo Fisher Scientific | 31,490 | – |
| Peptide, recombinant protein | WGA, Peroxidase (WGA-HRP) | Vector Laboratories | PL-1026-2 | – |
| Peptide, recombinant protein | IRDye 800CW Streptavidin | Li-Cor | 926-32230 | 1:10,000 |
| Peptide, recombinant protein | IRDye 680RD Donkey anti-Goat IgG | Li-Cor | 925-68074 | 1:10,000 |
| Commercial assay or kit | BCA assay | Thermo Fisher Scientific | 23228 | – |
| Commercial assay or kit | Preomics iST kit | PreOmics | P.O.00027 | – |
| Commercial assay or kit | Pierce quantitative colorimetric peptide assay | Thermo Fisher Scientific | 23275 | – |
| Commercial assay or kit | Expi293 media | Thermo Fisher Scientific | A1435101 | – |
| Commercial assay or kit | Keratinocyte SFM media (1×) | Thermo Fisher Scientific | 17005042 | – |
| Commercial assay or kit | MycoAlert PLUS mycoplasma detection kit | Lonza | LT07-703 | – |
| Chemical compound, drug | Trolox | Fisher Scientific | 501176131 | – |
| Chemical compound, drug | Sodium Ascorbate | Sigma-Aldrich | A4034-100G | – |
| Chemical compound, drug | N-acetyl-D-glucosamine | Sigma-Aldrich | A3286-5G | – |
| Chemical compound, drug | H2O2 (1×) | Sigma-Aldrich | H1009-100ML | – |
| Chemical compound, drug | Hemin-Cl | Sigma-Aldrich | 51280-1G | – |
| Chemical compound, drug | Maleimide DBCO | Click Chemistry Tools | A108P-10 | – |
| Chemical compound, drug | Biotin Tyramide | Sigma-Aldrich | SML2135-50MG | – |
| Chemical compound, drug | Sodium Pyruvate | Sigma-Aldrich | S8636-100ML | – |
| Chemical compound, drug | Sodium Periodate | Sigma-Aldrich | 311448-5G | – |
| Chemical compound, drug | Biocytin Hydrazide | Biotium | 90060 | – |
| Chemical compound, drug | Aniline | Sigma-Aldrich | 242284 | – |
| Chemical compound, drug | Protease Inhibitor Cocktail | Sigma-Aldrich | P8340-1ML | – |
| Chemical compound, drug | High Capacity Neutravidin Resin | Thermo Fisher Scientific | 29204 | – |
| Chemical compound, drug | Poly-D-lysine | Thermo Fisher Scientific | A3890401 | – |
| Chemical compound, drug | Sulfo NHS LC-LC Biotin | Thermo Fisher Scientific | A35358 | – |
| Software, algorithm | FlowJo | FlowJo (https://www.flowjo.com) | RRID:SCR_008520 | – |
| Software, algorithm | GraphPad Prism | Prism (https://www.graphpad.com/scientific-software/prism/) | RRID:SCR_002798 | – |
| Software, algorithm | Morpheus | Morpheus (https://software.broadinstitute.org/morpheus/) | – | – |
| Software, algorithm | FIJI | FIJI (https://fiji.sc) | RRID:SCR_002285 | – |
| Other | Penicillin/streptomycin | Thermo Fisher Scientific | 15-140-122 | – |
| Other | Fetal bovine serum | Gemini Bio-Products | 100-106 | – |
| Other | Trypsin (0.05%) | Life Technologies | 25300054 | – |