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. 2022 Mar 8;7(5):e155236. doi: 10.1172/jci.insight.155236

Figure 2. Age increases expression of IL-36α, IL-36β, and VCAM-1 in healthy and IR-injured hearts.

Figure 2

Hearts from adult and aged sham and IR-injured mice were immunostained with an anti–IL-36α/β, anti-CD31, and anti–VCAM-1 antibody. (A) Representative images of IL-36α (green; top 2 rows) and IL-36β (green; bottom 2 rows) staining of frozen heart sections costained with CD31 (red) and VCAM-1 (blue). The upper row of each cytokine panel shows coronary microvessels with the lower row selected to demonstrate staining of a large coronary blood vessel. Quantitative analysis of the immunofluorescence images for (B) IL-36α and (C) IL-36β expression. *P = 0.0193. **P = 0.0054. ****P < 0.0001. (D) Representative images of VCAM-1 (blue) staining of frozen heart sections. (E) Quantitative analysis of immunofluorescence images of VCAM-1 expression. *P = 0.0308. ***P = 0.0002. ****P < 0.0001. Scale bar indicates 200 μm. n = 4/group. P values were determined using a 1-way ANOVA followed by a Tukey’s post hoc test.