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. 2022 Mar 8;7(5):e155236. doi: 10.1172/jci.insight.155236

Figure 6. Age increases thromboinflammatory disturbances within the healthy and IR-injured beating heart coronary microcirculation in vivo, which can be prevented in both age groups by IL-36R inhibition.

Figure 6

An IL-36Ra (15 μg/mouse) was injected intra-arterially at 10 minutes prereperfusion and 60 minutes postreperfusion in adult and aged mice. (A) Representative intravital images of the beating heart showing adherent neutrophils (green) and platelets (red) in the coronary microcirculation at 60 and 120 minutes in sham hearts or 60 and 120 minutes postreperfusion in injured hearts. Adherent neutrophils (green) and platelet microthrombi (red) are primarily within coronary capillaries in injured hearts. Intensely fluorescent green areas in aged IR-injured hearts correspond to medium-sized blood vessels that have become delineated by the presence of a substantial number of adherent neutrophils. Quantitative analysis of the intravital data for adherent neutrophils in (B) adult and (C) aged IR-injured hearts and the AUC for (D) adherent neutrophils and (E) platelets over a time course of 150 minutes. Scale bar indicates 100 μm. Adult sham — n = 5/group; adult IRI — n = 6/group; adult IRI + IL-36Ra — n = 5/group; aged sham — n = 7/group; aged IRI — n = 5/group; aged IRI + IL-36Ra — n = 8/group. *P = 0.0339, **P = 0.0014, ***P = 0.0003, ****P < 0.0001 as determined using a 1-way ANOVA followed by a Tukey’s post hoc test.