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. 2022 Mar 8;7(5):e155260. doi: 10.1172/jci.insight.155260

Figure 7. PKM2 and its enzymatic activity in regulation of VEGF production in podocyte.

Figure 7

(A) Western blotting of PKM1 and PKM2 in pEmpty, phPKM1, phPKM2, phPKM2(K270M), pPKMKD, pPKMKD + phPKM1, pPKMKD + phPKM2, and pPKMKD + phPKM2(K270M) podocyte stable cell lines. (B) PK activity of pEmpty (n = 11), phPKM1 (n = 4), phPKM2 (n = 4), phPKM2(K270M) (n = 5), pPKMKD (n = 7), pPKMKD + phPKM1 (n = 9), pPKMKD + phPKM2 (n = 7), and pPKMKD + phPKM2(K270M) (n = 4) podocyte stable cell lines in basal level and 10 μM TEPP-46 treatment. *P < 0.05, **P < 0.01 versus pEmpty; #P < 0.05, ##P < 0.01 for each pair of the compared groups. (C and D) mRNA expression of Hif1a and Vegf in pEmpty, phPKM1, phPKM2, phPKM2(K270M), pPKMKD, pPKMKD + phPKM1, pPKMKD + phPKM2, and pPKMKD + phPKM2(K270M) lines. n = 3; *P < 0.05, **P < 0.01. #P < 0.05, ##P < 0.01 for each pair of the compared groups. (E) VEGF levels 24 hours in culture media of pEmpty, phPKM1, phPKM2, phPKM2(K270M), pPKMKD, pPKMKD + phPKM1, pPKMKD + phPKM2, and pPKMKD + phPKM2(K270M) lines. n = 4; **P < 0.01 versus pEmpty; ##P < 0.01 for each pair of the compared groups. (F) mRNA expression of mitochondrial-related genes in pEmpty, pPKMKD, pPKMKD + phPKM1, pPKMKD + phPKM2, and pPKMKD + phPKM2(K270M) lines. (G) mRNA expression of mitochondrial-related genes in pEmpty, phPKM1, phPKM2, and phPKM2(K270M) lines. n = 3; **P < 0.01 versus pEmpty; #P < 0.05, ##P < 0.01. Data are mean ± SEM, 2-way ANOVA followed by correction for multiple comparisons with Tukey’s post hoc test.