Figure 7. Atypical GCs in NZM2328 DKO mice.

(A) PNA⁺ GC clusters (original magnification, 10×) in NZM2328 mice (top) and PNA⁺ clusters without FDCs in DKO mice (bottom). (B). Percentage CD95⁺GL7⁺ B cells in CD19⁺ B cells in nephritic NZM and DKO mice. (C and D) CD95⁺GL7⁺ B cells include PNA^hiCD38^lo GC B cells and PNA^loCD38⁺ memory cells. (E) Flow plots show the gating of splenic CD95⁺GL7⁺ B cells in CD19⁺ B cells from NZM and DKO mice and their LZ and DZ phenotype. (F and G) Bar graphs show the summary result of GL7 expression (F) and LZ/DZ ratio (G). (H) Percentage CCR6⁺CD38⁺ memory B cells in LZ CD95⁺GL7⁺CXCR4^–CD86⁺ compartment in NZM and DKO mice. (I and J) Percentage (I) and number (J) of CCR6⁺CD38⁺ memory B cells in NZM and DKO mice. (K) Immunohistochemistry images (original magnification, 20×) show PD1^hiCD4⁺ T cells in DZ of GCs of NZM (top) and scattered throughout the GC cluster in DKO (bottom) mice. (L) Immunohistochemistry images (original magnification, 20×) show Ki67⁺Bcl6⁺ B cells in GCs of NZM (top) and DKO (bottom) mice (representative of 3–4 mice per group). (M) Pie charts show mutation frequencies in VH sequences from CD95⁺GL7⁺ B cells from NZM (left) and DKO (right) mice. χ², ***P < 0.001. (N and O) Percentage ANA-positive B cells in CD95⁺GL7⁺ GC B cells (N) and follicular IgM⁺IgD⁺ B cells (O). (P and Q) Bar graphs show the summary result of Bcl6 (P) and Bach2 (Q) expression on CD95⁺GL7⁺ GC B cells. NZM mice (white bars); DKO mice (gray bars). Dots on bar graphs represent individual mice. Immunohistochemistry representative of 3–5 mice per group. (B, D, F–J, N, and O) Mann-Whitney nonparametric t test, **P < 0.01, ***P < 0.001. (P and Q) ANOVA Kruskal-Wallis with Dunn’s multiple comparisons test, **P < 0.01.