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. 2022 Mar 29;2022:6036457. doi: 10.1155/2022/6036457

Figure 1.

Figure 1

Sensitivity and specificity assays of nested PCR. (a) The nested PCR electrophoresis map of different concentrations of H. pylori. The target band was 100 bp. The concentrations of H. pylori in wells 1~8 were 2 × 108 CFU/mL, 2 × 107 CFU/mL, 2 × 106 CFU/mL, 2 × 105 CFU/mL, 2 × 104 CFU/mL, 2 × 103 CFU/mL, 2 × 102 CFU/mL, and 2 × 101 CFU/mL. (b) The nested PCR electrophoresis map of different bacteria. The bacterial samples in wells 1-9 were H. pylori ATCC 43504, H. pylori SS1, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Klebsiella pneumonia CMCC 46114, Proteus mirabilis CMCC 49005, Campylobacter jejuni subsp. ATCC 700819, and nucleotide-free water. (c) The nested PCR electrophoresis map of different bacteria. The bacterial samples in wells 1-3 were nucleotide-free water, H. pylori ATCC 43504, and H. bilis ATCC51630.