Figure 5.

Aspirin decreases PD-L1 expression in OC cells via targeting KAT5. (a–c) The levels of KAT5 and PD-L1 in OC cells was checked by qPCR assay. (d–f) OC cells were treated with aspirin, and then ChIP assay was conducted to check enrichment of (d) KAT5, (e) H3K27ac, and (f) RNA polymerase II on promoter region of PD-L1. (g) ChIP assay to assess the enrichment of H3K27ac on promoter of PD-L1 in OC cells transfected with shKAT5. (h) The interaction of KAT5 with H3K27ac and RNA polymerase II on promoter region of PD-L1 was measured by ChIP-re-ChIP analysis. (i) QRT-PCR assay to check PD-L1 level in OC cells treated with aspirin alone or with KAT5 overexpressing plasmid. ∗∗P < 0.01.