Skip to main content
. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: J Immunol. 2022 Mar 18;208(7):1813–1827. doi: 10.4049/jimmunol.2100700

Figure 4. Binding Stability and Dissociation Constant are Significantly Correlated but Both Contribute to Immunogenicity.

Figure 4.

(A) Correlation between all calculated neoantigen characteristics: mRNA expression (Exp.), Variant Allele Frequency (VAF), proteasomal cleavage potential (Cle.), TAP transport potential (TAP), MHC class I to neoantigen dissociation constant (Kd), MHC class I to neoantigen binding stability (Stab.), TCR recognition probability (TCR), amplitude, defined as the ratio of the MHC class I binding of the closest matched human peptide and the neoantigen (Amp.), and normalized sequence similarity score for closest matched human peptide and the neoantigen (Sim.). The size of the circles indicates the absolute magnitude of the correlation. (B) Correlation of MHC class I dissociation constant with MHC class I binding stability of the neoantigen. Lines show the linear relationship between the characteristics for immunogenic (black) and non-immunogenic (gray) neoantigens, respectively. *:p<0.05