Fig. 1. Scheme of the experiment.

OVX – ovariectomy; GTT – glucose tolerance test; Chow – ad libitum access to commercial mouse chow; HSFD – high sweet fat diet (standard laboratory chow, sweet cookies, lard); Treatment – ovariectomized females with diet-induced obesity received fibroblast growth factor 21 (FGF21) at a dose of 1 mg per 1 kg of body weight subcutaneously and estradiol (E2) at a dose of 20 mg/animal orally separately or together for 13 days. Control females received subcutaneously phosphate buffered saline (PBS) and oil orally.