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. 2022 Mar 15;28:261–278. doi: 10.1016/j.omtn.2022.03.011

Figure 3.

Figure 3

NF1 protein expression and RAS activity

Functional analysis of mNf1 isoforms with selected exon skips. (A) Representative western blot of NF1 and tubulin levels. (B) Quantitation of NF1/tubulin ratios normalized to WT ratio; N ≥ 3. Error bars represent SEM. Resultant neurofibromin abundance for each cDNA was compared with WT levels by t test. Red asterisk indicates p < 0.05. (C) GTP-RAS levels normalized to WT and compared with EV control; N > 3. (D) Representative western blot of pERK/ERK ratios. (E) Quantitation of pERK/ERK ratios normalized to WT and compared with EV control; N ≥ 3. (F) ELK1 transcriptional activity normalized to WT and compared with EV control; N ≥ 3. Error bars represent SEM.