REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
osteological remain	this study	Att27
osteological remain	this study	Att3
osteological remain	this study	Att52-2
osteological remain	this study	BES1096B
osteological remain	this study	BES1154
osteological remain	this study	BES1249
osteological remain	this study	BFT228
osteological remain	this study	BLH447
osteological remain	this study	BPV1445
osteological remain	this study	BPV1455
osteological remain	this study	BUCH48-1
osteological remain	this study	BUCH48-2
osteological remain	this study	BUCH82
osteological remain	this study	CHF106
osteological remain	this study	CLR23
osteological remain	this study	CLR24
osteological remain	this study	CLR31
osteological remain	this study	CLR35
osteological remain	this study	CLR44
osteological remain	this study	Col239
osteological remain	this study	Col330
osteological remain	this study	CROI1-4
osteological remain	this study	CROI11
osteological remain	this study	CROI12-2
osteological remain	this study	ERS83-2
osteological remain	this study	GDF1231
osteological remain	this study	GDF1264
osteological remain	this study	GDF1341
osteological remain	this study	GDF1348
osteological remain	this study	GDF1349B
osteological remain	this study	GLN126A
osteological remain	this study	GLN126B
osteological remain	this study	GLN141
osteological remain	this study	GLN29-A
osteological remain	this study	GLN32
osteological remain	this study	Gox287
osteological remain	this study	ISL6950
osteological remain	this study	PAL170
osteological remain	this study	Pech3
osteological remain	this study	Pech9
osteological remain	this study	PEY73
osteological remain	this study	PT7
osteological remain	this study	SCPG2
osteological remain	this study	UN129
osteological remain	this study	UN19
osteological remain	this study	UN85
osteological remain	this study	VAS75
osteological remain	this study	VAS79-2
osteological remain	this study	WET429
Chemicals, peptides, and recombinant proteins
Proteinase K 100MG	Sigma Aldrich	Cat# 3115879001
Buffer QG	Qiagen	Cat#19063
OneTaq® 2X Master Mix with Standard Buffer	New England Biolabs	Cat# M0482L
Phusion® High-Fidelity DNA Polymerase	New England Biolabs	Cat# M0530L
NEBNext End Repair Module	New England Biolabs	Cat# E6050L
NEBNext Quick Ligation Module	New England Biolabs	Cat# E6056L
USER Enzyme	New England Biolabs	Cat# M5505L
Critical commercial assays
MinElute PCR Purification kit	QIAGEN	Cat# 28006
Qubit dsDNA HS Assay Kit	Thermo Fisher Scientific	Cat# Q32854
NextSeq 500/550 High Output Kit v2.5 (150 Cycles)	Illumina	Cat# 20024907
Agilent High Sensitivity DNA Kit	Agilent	Cat# 5067-4627
Deposited data
Raw and analyzed data	This study	ENA Project: PRJEB50940
Software and algorithms
READ	Monroy Kuhn et al., 2018	https://bitbucket.org/tguenther/read/src/master/
Admixtools	Patterson et al., 2012	https://github.com/DReichLab/AdmixTools
Yleaf	Ralf et al., 2018	https://github.com/genid/Yleaf
Haplogrep	Dür et al., 2021	https://github.com/seppinho/haplogrep-cmd
ANGSD	Korneliussen et al., 2014	https://github.com/ANGSD/angsd
smartPCA		https://github.com/DReichLab/EIGtree/master/POPGEN
bamUtil		bamUtil 71 https://github.com/statgen/bamUtil
EAGER	Peltzer et al., 2016	https://github.com/apeltzer/eager-gui
AdmixR	Petr et al., 2019	https://github.com/bodkan/admixr