Figure 2.
KIBRA Knockout stabilizes intracellular Aβ/AβPP in 5XFAD mice. (a and b) Representative images and quantification analysis of cortical and hippocampal regions of the indicated 3–5-month-old mice. Intraneuronal Aβ/AβPP in the brain sections were stained with the anti-4G8 antibody (green color), and neuronal nuclear with the DAPI (blue color). Scale bar = 200 µm in upper panel, 50 µm in lower panel. (b-c) Quantification analysis of the same cortical (b) and hippocampal regions (c) of indicated 3–5-month-old mice, respectively. More than seven mice per group were analyzed. (d) WB and quantification analysis of cell lysates from the KIBRA-KD and CTRL HT22 cells with APPswe overexpression for APP/APP-CTFβ (anti-C1/6.1). (e) Quantification analysis of Aβ40 and Aβ42 levels in cell lysates from the KIBRA-KD and CTRL HT22 cells with APPswe overexpression by Simoa technique. (n = 3, from three independent experiments). Data are represented as the mean ± SE. n.s.≥0.05 was determined by one-way ANOVA followed by Tukey's post hoc comparisons tests (b-c) or two-tailed Student's t test (d-e).