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. 2022 Apr 1;78:103980. doi: 10.1016/j.ebiom.2022.103980

Figure 4.

Fig 4

KIBRA knockout causes increases in number of MVBs harboring AβPP/Aβ. (a and b) Representative electron microscopic images of the hippocampus (a) and cortex (b) area in KIBRA−/−5XFAD and 5XFAD mice. Scale bar = 500 nm. Red arrows indicate MVBs containing typical ILVs. Quantification analysis of the number of MVBs per cell profile and the number of ILVs per MVB. ILVs and MVBs in 28-31 profiles of different cells were counted in a blind manner and only MVBs containing typical ILVs were counted. (c) Confocal microscopy analysis of Aβ/AβPP (anti-Aβ, clone 4G8, red) with CD63 (green) in brain slices of KIBRA−/−5XFAD and 5XFAD mice. Scale bar = 10 µm. Right panel represents overlap coefficient per cell. Data are represented as the mean ± SE. *P < 0.05, ⁎⁎⁎P < 0.001, and ⁎⁎⁎⁎P < 0.0001 in two-tailed Student's t test.