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| Tube | Detail | Replicates |
|---|---|---|
| Live Dead control | Heat-kill cells in a 70°C water bath, for 10 min | 1 |
| Controls | CD4 FMO recommended as infection decreases CD4 expression | 1 |
| Non-stained control | 1 | |
| Test Samples | Uninfected control CD4-onlya | 1 |
| Infected CD4+ targets | 6b | |
| Co-culture: Infected CD4+ targets + Effector CD8+ | 6b |
