Table 3. Minimum required percentage of p24⁺ cells for 90% statistical power.

At 90% statistical power, the minimum percentage of p24⁺ cells (in HIV-CD4 T cell cultures) needed to observe a threshold virus inhibition of 5–30% for 2–6 replicates. The threshold of 8% inhibition is the receiver operating characteristic (ROC)-determined cut-off of specific CD8⁺ T cell-mediated virus inhibition from study data. Percentages of p24⁺ cells were generated using simulations (500 iterations) based on parameters from the real data. The minimum allowed percentage of HIV infection was 0.5%.

	Virus inhibition (%)
Number of replicates	5	8	10	15	20	25	30
2	14.99	7.49	4.78	1.98	1.05	0.61	0.5
3	11.84	4.63	3.16	1.3	0.67	0.5	0.5
4	8.91	3.69	2.3	0.95	0.5	0.5	0.5
5	7.79	3.17	1.88	0.79	0.5	0.5	0.5
6	6.97	2.43	1.57	0.66	0.5	0.5	0.5